LC-NMR - Expanding the Limits of Structure Elucidation

LC-NMR: Expanding the Limits of Structure Elucidation (ChromatographicScience Series)
LC-NMR: 扩展的极限结构说明(色谱科学系列)
by Nina C. Gonnella ISBN: 1439875065 | 2013 | PDF | 301 pages | 4.8 MB

The isolation and structural characterization ofsubstances present at very low concentrations, as is necessary to satisfyregulatory requirements for pharmaceutical drug degradants and impurities, canpresent scientific challenges. The coupling of HPLC with NMR spectroscopy hasbeen at the forefront of cutting-edge technologies to address these issues.LC-NMR: Expanding the Limits of Structure Elucidation presents a comprehensiveoverview of key concepts in HPLC and NMR that are required to achieve definitivestructure elucidation with very low levels of analytes. Because skill sets fromboth of these highly established disciplines are involved in LC-NMR, the authorprovides introductory background to facilitate readers’ proficiency in bothareas, including an entire chapter on NMR theory.

This book provides guidance in setting up LC-NMRsystems, discussion of LC methods that are compatible with NMR, and an updateon recent hardware and software advances for system performance, such asimprovements in magnet design, probe technology, and solvent suppressiontechniques that enable unprecedented mass sensitivity in NMR. It also describesnumerous NMR collection strategies, including continuous flow, stop flow, solidphase extraction (SPE), loop collection, and c*挨批唉*llary electrophoresis. Inaddition, the author presents an overview of NMR experiments and techniquesused in structure elucidation.

The text focuses on current developments inchromatographic-NMR integration, with particular emphasis on utility in thepharmaceutical industry. Applications include trace analysis, analysis ofmixtures, and detection of degradation products, impurities, metabolites,peptides, and more. The text discusses novel uses and emerging technologiesthat challenge detection limits as well future directions for this importanttechnique. This book is a practical primary resource for NMR structuredetermination—including theory and application—that guides the reader throughthe steps required for isolation and NMR structure elucidation on the microscale.